dc.contributor.author | Monsigny, M. | fr_FR |
dc.contributor.author | Midoux, P. | fr_FR |
dc.contributor.author | Roche, A.C. | fr_FR |
dc.date.accessioned | 2013-02-18T16:18:05Z | |
dc.date.available | 2013-02-18T16:18:05Z | |
dc.date.issued | 1993 | fr_FR |
dc.identifier.citation | Monsigny, M. ; Midoux, P. ; Roche, A.C., Perspectives ex vivo et in vivo pour la thérapie génique, de la transfection sélective à l'aide de complexes plasmide-polylysine ciblés, Med Sci (Paris), 1993, Vol. 9, N° 4; p.441-449 | fr_FR |
dc.identifier.issn | 1958-5381 | fr_FR |
dc.identifier.uri | http://hdl.handle.net/10608/2938 | |
dc.description.abstract | Gene therapy will be an ideal method when it is possible to transfer DNA in cells with a great efficacy and an absolute safety in vivo. Along with viral carriers which are efficient but may not be strictly safe, non viral carriers could be advantageously used. Polylysine, a polycation giving stable DNA complexes, is proposed as the basis of such a carrier system. Indeed, polylysine substituted with either a protein easily taken up by cells due to specific cell surface receptors, or carbohydrate moieties which selectively interact with lectins expressed at the surface of specific cells, allows a cell specific delivery of genes. In addition, when such a targeted carrier is used together with components that protect the plasmid- targeted polylysine complex from the hydrolytic activities of lysosomes and/or help the complex to cross the cell membranes to reach the cytosol, the specificity and the efficacy of the transfection are conspicuously higher. | fr |
dc.language.iso | fr | fr_FR |
dc.publisher | John Libbey Eurotext, Montrouge | fr_FR |
dc.rights | Article en libre accès | fr |
dc.rights | Médecine/Sciences - Inserm - SRMS | fr |
dc.source | M/S. Médecine sciences [revue papier, ISSN : 0767-0974], 1993, Vol. 9, N° 4; p.441-449 | fr_FR |
dc.title | Perspectives ex vivo et in vivo pour la thérapie génique, de la transfection sélective à l'aide de complexes plasmide-polylysine ciblés | fr |
dc.type | Article | fr_FR |
dc.identifier.doi | 10.4267/10608/2938 | |