dc.contributor.author | Nemoz-Billet, Laurie | - |
dc.contributor.author | Bretaud, Sandrine | - |
dc.contributor.author | Ruggiero, Florence | - |
dc.date.accessioned | 2022-10-18T07:10:35Z | |
dc.date.available | 2022-10-18T07:10:35Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Nemoz-Billet, Laurie ; Bretaud, Sandrine ; Ruggiero, Florence ; Le rôle de la matrice extracellulaire dans la régénération des nerfs moteurs, Med Sci (Paris), Vol. 37, N° HS ; p. 11-14 ; DOI : 10.1051/medsci/2021183 | |
dc.identifier.issn | 1958-5381 | |
dc.identifier.uri | http://hdl.handle.net/10608/11969 | |
dc.description.abstract | The motor neurons (MN) form the ultimate route to convey the commands from the central nervous system to muscles. During development, MN extend axons that follow stereotyped trajectories to their muscle targets, guided by various attractive and repulsive molecular cues. Extracellular matrix (ECM) is a major source of guidance cues, but its role in axonal development and regeneration remains poorly documented. Regenerating axons are able to return to their synaptic target following their original trajectory. The same guidance cues could be thus involved in motor nerve regeneration. Zebrafish has become a popular model system in understanding the development of the peripheral nervous system. Thanks to the generation of fluorescent transgenic lines and the optical transparency of embryos and larvae, it allows direct visualization of axonogenesis. Additionally, and contrary to humans, its remarkable capacity to regenerate makes it well suited for the study of nerve regeneration. A laser method to ablate nerves in living zebrafish larvae has been developed in our laboratory that, combined with the use of the fluorescent
mnx1:gfp
zebrafish transgenic line, allows the follow up of the dynamics of the nerve regeneration process. To study the role of ECM proteins present in the axonal path, mutant lines for different ECM proteins (already available in our laboratory or generated in
mnx1:gfp
fish using CRISPR-Cas9 method) will be used to analyze their role during the regeneration process. These mutant lines for ECM will be crossed with existing fluorescent transgenic lines to visualize different cell types involved in the nerve regeneration, such as macrophages (
mfap4:mcherry
), neutrophils (
mpx:gfp
) or even Schwann cells (
sox10:mrfp
). Overall, this study will depict the role of ECM in nerve regeneration and will provide essential knowledge for the development of new biomaterials to promote the regeneration of injured motor nerves. | en |
dc.language.iso | fr | |
dc.publisher | EDP Sciences | |
dc.relation.ispartof | Prix SFM | |
dc.rights | Article en libre accès | fr |
dc.rights | Médecine/Sciences - Inserm - SRMS | fr |
dc.rights.uri | | |
dc.source | M/S. Médecine sciences [ISSN papier : 0767-0974 ; ISSN numérique : 1958-5381], Vol. 37, N° HS; p. 11-14 | |
dc.subject.mesh | Animaux | fr |
dc.subject.mesh | Animal génétiquement modifié | fr |
dc.subject.mesh | Axones | fr |
dc.subject.mesh | Protéines de transport | fr |
dc.subject.mesh | Matrice extracellulaire | fr |
dc.subject.mesh | Protéines de la matrice extracellulaire | fr |
dc.subject.mesh | Glycoprotéines | fr |
dc.subject.mesh | Protéines à homéodomaine | fr |
dc.subject.mesh | Humains | fr |
dc.subject.mesh | Larve | fr |
dc.subject.mesh | Régénération nerveuse | fr |
dc.subject.mesh | Cellules de Schwann | fr |
dc.subject.mesh | Facteurs de transcription | fr |
dc.subject.mesh | Danio zébré | fr |
dc.title | Le rôle de la matrice extracellulaire dans la régénération des nerfs moteurs | fr |
dc.title.alternative | The role of extracellular matrix in the regeneration of motor nerves | en |
dc.type | Article | |
dc.contributor.affiliation | Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, ENS de Lyon, CNRS UMR 5242 , 46 allée d’Italie , Lyon F-69364 , France | |
dc.identifier.doi | 10.1051/medsci/2021183 | |
dc.identifier.pmid | 34878386 | |